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KMID : 0861120140180030117
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Korean Journal of Oriental Preventive Medicine
2014 Volume.18 No. 3 p.117 ~ p.128
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The effects of Pueraria lobata extract on gene expression in liver tissue of rat with estrogen-deficient obesity
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Shin Yoon-Sang
Hwang Bang-Yeon
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Abstract
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Objective : It is known that Pueriaria lobata has an anti-osteoporetic effect, anti-cancer effect, anti-pyreticeffect, and anti-diabetic effect. The aim of this study was to evaluate anti-obesity effect of Pueriaria lobataextract (PLE), and elucidate the effect of it on gene expression related to lipid metabolism.
Method : The experiments were performed with the use of ovariectomized rats as estrogen-deficient obesitymodel. They were grouped NC (normal control), OC (estrogen-deficient control), PLH (100mg/kg of PLE),PLL (20mg/kg). PLE was orally administered for 6 weeks. Body weights and serum lipid level were estimated,and real-time PCR was performed to investigate the effect of PLE on gene expression in liver.
Results : PLE decreased the body weight and serum cholesterol and triglyceride, but increased HDL-cholesterol. And PLE increased leptin, CYP27, CPT1, CYP8B1, ACAT2, LDLR, and SCD1, but reduced PPARγ, PGC1A,HMG-CoA-R, ACAT1, SCD1, and APoB gene expression in liver tissue of rat with estrogen-deficient obesity.
Conclusion : It is concluded that Pueriaria lobata reduced body weight, and its effect was expressed byregulation of gene expression related to lipid metabolism in rats with estrogen-deficient obesity.
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KEYWORD
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Pueriaria lobata, obesity, estrogen, gene expression
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